This study introduces a rotationally-driven dynamic solid phase sodium bisulfite conversion disc for forensic epigenetic sample preparation.
Epigenetic analysis requires sodium bisulfite conversion (BSC), a sample preparation method that is time-consuming, labor-intensive, prone to contamination, and characterized by DNA loss and fragmentation. To provide an alternative method for BSC that is more amenable to integration with the forensic DNA workflow, the authors of this study describe a rotationally-driven, microfluidic method for dynamic solid phase-BSC (dSP-BSC) that streamlines the sample preparation process in an automated format, capable of preparing up to four samples in parallel. The method permitted decreased incubation intervals by ∼36% and was assessed for relative DNA recovery and conversion efficiency and compared to gold-standard and enzymatic approaches. (Published Abstract Provided)
Downloads
Similar Publications
- Structuring Justice: How Prosecutorial Offices Handle Hate Crime Detection and Prosecution
- Do the criminal histories of vacant properties matter? Evidence from demolition and rehab interventions in Cleveland, Ohio
- Subadult Age Estimation Using the Mixed Cumulative Probit and a Contemporary United States Population